Scientists at the University of Colorado demonstrated a multi-photon-spatial frequency modulation imaging (MP-SPIFI) technique with a spatial resolution of 2η (η is the most advanced non-linear light intensity unit) that breaks through the resolution limit of optical microscopy. Super-resolution microscopy has won the 2014 Nobel Prize in Chemistry for overcoming the diffraction limit, but requires precise control of the diffraction of a single fluorescent molecule within the limits. Researchers consider another well-established deep tissue imaging technique called multiphoton microscopy, which provides sample information that standard super-resolution techniques can not provide. For the first time in a paper published in the Proceedings of the National Academy of Sciences of the United States, the researchers demonstrated for the first time that superphotorescence can be achieved with multiphoton fluorescence and with secondary synchronous harmonics. When used in combination, the two photons are quenched to a double Frequency of photons. They also developed a dedicated multiphoton-spatial frequency modulation imaging microscope to HeLa cells and cadmium telluride solar cells as a sample, through fluorescence and second harmonic simultaneously collected image information, resulting in nanoscale images, the spatial resolution of 2η , More than the traditional multiphoton microscope. In traditional multiphoton microscopes, ultrashort laser pulses accumulate on the sample as a compact spot that excites the fluorescence to produce an image. The multiphoton-space-frequency modulation imaging microscope uses multiple femtosecond laser pulses to illuminate a large optical zone to interfere with each other to create an image. Another important advantage of multiphoton-spatial frequency modulation imaging microscopy is the ability to provide super-resolution imaging of highly dispersed biological tissues. Most of the super-resolution technology to cell immobilization in the glass, it is not suitable for living tissue. The new technology can be used for living tissue or larger biological tissue samples. The researchers pointed out that if you can increase the image resolution collected from living tissue samples, combined with a variety of comparison mechanisms, access to a large number of biological information. This result breaks the limits of existing optical microscopy and allows the observation of the physiological processes of individual cells in living tissue with unprecedented resolution. 30-40Nm³/H Oxygen Generator,Oxygen Generators With Inbuilt Compressor,Psa Oxygen Generator With Compressor,Oxygen Generator To Fill Tanks Jiangsu Minnuo Group Co.,Ltd , https://www.mingngroupcn.com
American scientists breakthrough optical microscopy resolution limit